Identification of mRNA for 5 - HT and 5 - HT receptor subtypes in human 1 2 coronary arteries
نویسندگان
چکیده
Objective: Although pharmacological studies have indicated that serotonin (5-HT)-evoked contraction of the human coronary artery is mediated by 5-HT -like and 5-HT receptors, the gene expression of 5-HT receptors is still unclear. We examined mRNA expression of 1 2 5-HT and 5-HT receptor subtypes in human coronary arteries. Methods: Total RNA was extracted from human coronary arteries of 14 1 2 patients at autopsy by the guanidine method. Reverse transcription-polymerase chain reaction (RT–PCR) and ribonuclease protection assays were performed to identify 5-HT and 5-HT receptor mRNA expression in human coronary artery. Results: By RT–PCR, 1 2 5-HT , 5-HT and 5-HT mRNAs were detected in all of the 14 patients. 5-HT , 5-HT , and 5-HT mRNAs were detected in only 1B 2A 2B 1A 1D 1E some patients. However, neither 5-HT mRNA nor 5-HT mRNA was detected in any patient. By ribonuclease protection assay, 1F 2C 5-HT and 5-HT signals were detected in all patients examined, but neither 5-HT , 5-HT nor 5-HT signal was detected in any 1B 2A 1A 1D 2B patient. Conclusions: Of 5-HT receptor subtypes, 5-HT and 5-HT receptor mRNAs were predominantly expressed in human 1 / 2 1B 2A coronary arteries. Our finding provides molecular evidence that the 5-HT receptor may be the 5-HT -like receptor which mediates 1B 1 constriction of human coronary arteries. 1999 Elsevier Science B.V. All rights reserved.
منابع مشابه
Variable Participation of 5-HT1-Like Receptors and 5-HT2 Receptors in Serotonin-Induced Contraction of Human Isolated Coronary Arteries
Background Serotonin may contract human large coronary arteries through two 5-hydroxtryptamine (5-HT) receptors, 5-HT1-like and 5 -HT2. These 5-HT1-like receptors resemble both cloned 5-HT1D receptor subtypes, 5-HT'1D, and 5 -HT1Ds. Although these subtypes have similar pharmacology, 5-HT1Dg receptors appear to have lower affinity for ketanserin than 5-HT1Di receptors. We assessed the relative p...
متن کاملParticipation of 5-HT1-Like Receptors and 5-HT2 Receptors in Serotonin-Induced Contraction of Human Isolated Coronary Arteries 5-HT1-Like Receptors Resemble Cloned 5-HT1Dp Receptors
Background Serotonin may contract human large coronary arteries through two 5-hydroxtryptamine (5-HT) receptors, 5-HT1-like and 5 -HT2. These 5-HT1-like receptors resemble both cloned 5-HT1D receptor subtypes, 5-HT'1D, and 5 -HT1Ds. Although these subtypes have similar pharmacology, 5-HT1Dg receptors appear to have lower affinity for ketanserin than 5-HT1Di receptors. We assessed the relative p...
متن کاملVariable participation of 5-HT1-like receptors and 5-HT2 receptors in serotonin-induced contraction of human isolated coronary arteries. 5-HT1-like receptors resemble cloned 5-HT1D beta receptors.
BACKGROUND Serotonin may contract human large coronary arteries through two 5-hydroxytryptamine (5-HT) receptors, 5-HT1-like and 5-HT2. These 5-HT1-like receptors resemble both cloned 5-HT1D receptor subtypes, 5-HT1D alpha and 5-HT 1D beta. Although these subtypes have similar pharmacology, 5-HT1D beta receptors appear to have lower affinity for ketanserin than 5-HT1D alpha receptors. We assess...
متن کاملSerotonin-induced hypercontraction through 5-hydroxytryptamine 1B receptors in atherosclerotic rabbit coronary arteries.
BACKGROUND Augmented vasoconstriction to serotonin (5-hydroxytryptamine [5-HT]) in atherosclerotic vessels plays a crucial role in the development of myocardial ischemia. We investigated mechanisms for serotonin-evoked hypercontraction in atherosclerotic rabbit coronary arteries. METHODS AND RESULTS Contractile responses to serotonergic agents of endothelium-denuded coronary arteries from con...
متن کاملSingle cell laser dissection with molecular beacon polymerase chain reaction identifies 2A as the predominant serotonin receptor subtype in hypoglossal motoneurons.
We hypothesize that sleep state-dependent withdrawal of serotonin (5-hydroxytryptamine, 5-HT) at upper airway (UAW) dilator motoneurons contributes significantly to sleep-related suppression of dilator muscle activity in obstructive sleep apnea. Identification of 5-HT receptor subtypes involved in postsynaptic facilitation of UAW motoneuron activity may provide pharmacotherapies for this preval...
متن کامل